科学研究情况简介 |
【近5年来主要研究课题】 1.新型酶资源开发:基因组采矿技术等 2.酶分子创制:定向改造CALB、PanD、LAAD、PDC等 3.医药中间体的高效制备:β-丙氨酸、α-羟基酮、苯丙酸等 【近5年来主持项目】 1.国家自然科学基金面上项目:金属离子调控Y家族聚合酶Dpo4合成能力和保真性的机制(21576117),负责人 2.国家自然科学基金面上项目:酸性磷酸酶底物识别机制及其磷酸化功能强化的研究(21878126),负责人 3.江苏省社会发展项目:微生物制备饲料添加剂维生素C磷酸酯的关键技术研究(BE2015307),负责人 4.企业合作项目:生物法生产一种糖胺聚糖硫酸盐的关键技术,2018-2020,负责人 【近5年来代表性论文】 1.Qian Y, Lu C, Liu J, Song W, Chen X, Luo Q, Liu L,Wu J*.Engineering protonation conformation of L-aspartate-α-decarboxylase to relieve mechanism-based inactivation.Biotechnol Bioeng. 2020,117:1607-1614 2.Yao R, Zhou P, Wu C, Liu L,Wu J*. Sml1 inhibits DNA repair activity of Rev1 in Saccharomyces cerevisiae during oxidative stress. Appl Environ Microbiol. 2020, 86(7). pii: e02838-19. doi: 10.1128/AEM.02838-19 3. Zhang Q, Yao R, Chen X, Liu L, Xu S, Chen J,Wu J*. Enhancing fructosylated chondroitin production in Escherichia coli K4 by balancing the UDP-precursors. Metab Eng. 2018,47:314-322 4.Yao R, Shi L, Wu C, Qiao W, Liu L,Wu J*. Lsm12 mediates Polη deubiquitination to help Saccharomyces cerevisiae resist oxidative stress. Appl Environ Microbiol. 2018,13;85(1). pii: e01988-18. 5.5. Wang L, Liang C,Wu J*, Liu L, Tyo KEJ. Increased Processivity, Misincorporation, and Nucleotide Incorporation Efficiency in Sulfolobus solfataricus Dpo4 Thumb Domain Mutants. Appl Environ Microbiol. 2017,83(18). pii: e01013-17. 6. Zheng K, Song W, Sun A, Chen X, Liu J, Luo Q,Wu J*. Enzymatic Production of Ascorbic Acid-2-phosphate by Recombinant Acid Phosphatase. J Agric Food Chem. 2017, 65(20):4161-4166. 7.Wu J, de Paz A, Zamft BM, Marblestone AH, Boyden ES, Kording KP, Tyo KEJ. DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase. Sci Rep. 2017,7(1):4756. 8.Wu J, Fan X, Liu J, Luo Q, Xu J, Chen X. Promoter engineering of cascade biocatalysis for α-ketoglutaric acid production by coexpressing L-glutamate oxidase and catalase. Appl Microbiol Biotechnol. 2018 ,102(11):4755-4764. 9. Song W, Xu X, Gao C, Zhang, Y,Wu J, Liu J, Chen X, Luo Q, Liu L .Open Gate of Corynebacterium glutamicum Threonine Deaminase for Efficient Synthesis of Bulky α‑Keto Acids,ACS Catalysis,2020, 10, 17, 9994–10004 10.Song W, Wang J,Wu J, Liu J, Chen X, Liu L. Asymmetric assembly of high-value α-functionalized organic acids using a biocatalyticchiral-group-resetting process. Nat Commun., 2018, 9:3818-3827. 【近5年主要授权专利】 1.Jing Wu, Quan Zhang, Xiulai Chen, Jia Liu, Qiuling Luo. A recombinant Escherichia coli for high efficiency production of fructosylated chondroitin and method for making thereof. Patent No.:US10508279B2. 2019-12-17 2.Jing Wu, Li Wang, DNA Polymerase with Increased DNA Catalytic Synthesis Extension Capacity Technical Field, PCT 16/198,773 3.Liming Liu, Wei Song,Jing Wu, Kai Zheng, Xiulai Chen. Method for preparing ascorbic acid-2-phosphate using a recombinant strain. Patent No.:Us 10,308,919 B2 4.吴静,王立.一种催化DNA合成延伸能力提高的DNA聚合酶.中国发明专利, ZL201610945974.6 5.吴静,云建荣,姚瑞.一种检测刀额新对虾特异性免疫球蛋白E方法,中国发明专利, ZL201710623119.8 6.吴静,云建荣,姚瑞.一种检测中华绒螯蟹特异性免疫球蛋白E方法,中国发明专利, ZL 201710786280.7 7.吴静,宋伟,郑凯.一种高产维生素C-2-磷酸酯的方法,专利号:ZL201610793043.9 8.吴静,梁晨晨.一种提高胰岛素前体产量的方法.授权号:ZL201611226771.8 【主要奖励】 1.酸性功能多糖发酵生产与结构调控的关键技术及应用.高等学校科学研究优秀成果奖科学技术进步奖二等奖(2/8),2018 2.微生物发酵生产酸性功能多糖的关键技术与应用.中国轻工业联合会科技进步奖二等奖, (2/7),2018 3.江苏省优秀硕士学位论文1篇, 2018 4.江南大学“至善青年学者”A类(2020-2021) |