科学研究情况简介
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【近年来获得的学术科研成果】 采用生物化学、合成生物学、代谢工程及酶工程等技术手段,解析甾体药物中间体、多糖、抗生素及维生素等的生物合成机制,调控优化代谢途径及发酵过程,构建高效合成微生物细胞工厂,实现其绿色合成。近年来共主持国家自然科学基金、江苏省自然科学基金、中国博士后面上项目基金及重点参与“863”重大项目子课题各1项;在国内外权威期刊发表科研论文50余篇;申请发明专利39件,授权专利15件;获得省部级科研奖励2项。 【近年来主要研究课题】 [1] 亚麻刺盘孢双羟化甾体化合物DHEA的分子机制研究,中国博士后基金面上项目一等(2018M640452),2018.11-2019.12. 主持. [2] 胶质芽孢杆菌胞外多糖BMPS的生物合成途径解析及其调控, 国家自然科学基金青年基金(31300026), 2014-2016, 主持. [3] 微生物胞外多糖的代谢途径解析与调控研究, 江苏省自然科学基金青年基金(BK2012117), 2012-2015, 主持. [4] 甾体药物中间体三羟基雄甾烯酮的高效生物转化机理及调控研究, 中央高校自主科研项目(JUSRP111A50), 2013-2015, 主持. [5] 甾体药物中间体生物转化及清洁生产技术, 863重大项目子课题(2011AA02A211-05), 2011-2016, 主要参与.
【近年来主要论文代表作】 [1] Li H, Wang XD, Zhou LF, Ma Y, Yuan WJ, Zhang XM, Shi JS, Xu ZH. Enhancing expression of 3-ketosteroid-9α-hydroxylase oxygenase, an enzyme with broad substrate range and high hydroxylation ability, in Mycobacterium sp. LY-1, Applied Biochemistry and Biotechnology. 2018, DOI:10.1007/s12010-018-2876-2. [2] Sun J, Li H*, Ni Y, Zhang XM, Shi JS, Xu ZH.Genome shuffling of Colletotrichum lini for improving 3β,7α,15α-trihydroxy-5-androsten-17-one production from dehydroepiandrosterone. Journal of Industrial Microbiology and Biotechnology, 2017, 44(6):937-947. [3] Li H, Wang XD, Ma Y, Yang NN, Zhang XJ, Xu ZH, Shi JS. Purification and characterization of a glycosidase with hydrolyzing multi-3-O-glycosides of spirostanol saponin activity from Gibberella intermedia. Journal of Molecular Catalysis B: Enzymatic, 2016, 128: 46-51. [4] Li H, Yin SQ, Zhang XM Zhang XJ, Li H, Shi JS, Xu ZH. Enhanced 3β,7α,15α-trihydroxy-5-androsten-17-one production from dehydroepiandrosterone by Colletotrichum lini ST-1 resting cells with Tween-80. Applied Biochemistry and Biotechnology, 2016, 178(1):91-100. [5] Li H, Fu ZZ, Zhang XM, Li H, Shi JS, Xu ZH. The efficient production of 3β,7α,15α-trihydroxy-5-androsten-17-one from dehydroepiandrosterone by Gibberella intermedia. Applied Biochemistry and Biotechnology, 2014, 174(8):2960-2971. [6] Li H, Fu ZZ, Li H, Dou WF, Shi JS, Xu ZH. Improvement of the steroid dihydroxylation efficiency from dehydroepiandrosterone using a substrate pre-induction biotransformation process. Biotechnology and Bioprocess Engineering, 2013, 18(3):486-490. [7] Li H, Xu H, Li S, Feng XH, Ouyang PK. Optimization of exopolysaccharide welan gum production by Alcaligenes sp. CGMCC2428 with Tween-40 using response surface methodology. Carbohydrate Polymers, 2012, 87(2):1363-1368. [8] Su C, Gong JS, Sun YX, Qin JF, Zhai S, Li He, Li H, Lu ZM, Xu ZH, Shi JS. Combining pro-peptide engineering and multisite saturation mutagenesis to improve the catalytic potential of keratinase. ACS Synthetic Biology, 2019, 8(2): 425-433. [9] Tao LY, Gong JS, Su C, Jiang M, Li H, Li H, Lu ZM, Xu ZH and Shi JS. Mining and Expression of a Metagenome-Derived Keratinase Responsible for Biosynthesis of Silver Nanoparticles. ACS Biomaterials Science and Engineering, 2018, 4(4):1307-1315. [10] Zhang XJ, Liu YY, Li H, Su LL, Zhou LF, Peng J, Shen R, Zhang Y, Shi JS, Xu ZH. Microemulsion system for Colletotrichum lini ST-1 biotransformation of dehydroepiandrosterone to 7α,15α-diOH-DHEA. Biochemical Engineering Journal, 2018, 131:77-83. [11] Li C, Li H, Sun J, Zhang XY, Shi JS, Xu ZH. Production of 7α,15α-diOH-DHEA from dehydroepiandrosterone by Colletotrichum lini ST-1 through integrating glucose-feeding with multi-step substrate addition strategy. Bioprocess and Biosystems Engineering, 2016, 39(8):1-8. [12] Shao ML, Zhang X, Rao ZM, Xu MJ, Yang TW, Li H, Xu ZH, Yang ST. Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration. Green Chemistry, 2015, 18(6):1774-1784. [13] Wu Y, Li H, Zhang XM, Gong JS, Li H, Rao ZM, Shi JS, Xu ZH. Improvement of NADPH-dependent P450-mediated biotransformation of 7α,15α-diOH-DHEA from DHEA by a dual cosubstrate-coupled system. Steroids, 2015, 101:15-20. [14] Wu Y, Li H, Zhang XM, Gong JS, Rao ZM, Shi S, Xu ZH. Efficient hydroxylation of functionalized steroids by Colletotrichum lini ST-1. Journal of Molecular Catalysis B: Enzymatic, 2015, 120:111-118. [15] Shao ML, Zhang X, Rao ZM, Xu MJ, Yang TW Li H, Xu ZH. Enhanced production of androst-1, 4-Diene-3, 17-Dione by Mycobacterium neoaurum JC-12 using three-stage fermentation strategy. PLoS one, 2015, 10(9):e0137658. [16] Li H, Fu ZZ, Li H, Zhang XM, Shi JS, Xu ZH. Enhanced biotransformation of dehydroepiandrosterone to 3β,7α,15α-trihydroxy-5-androsten-17-one with Gibberella intermedia CA3-1 by natural oils addition. Journal of Industrial Microbiology and Biotechnology, 2014, 41(10):1497-1504.
【主要授权国家发明专利】 [1] 一株高效转化黄姜中皂苷生产薯蓣皂苷元的菌株及其应用.(授权号, ZL201210167132.4) [2] 一株高效转化去氢表雄酮菌株及其应用.(授权号, ZL201110438752.2) [3] 一种利用亚麻刺盘孢霉羟化去氢表雄酮的方法.(授权号, ZL2012104167406.X ) [4] 一种高效利用亚麻刺盘孢霉合成7α-羟基-雄甾烯酮的方法. (授权号, ZL201310570312.1 ) [5] 一种7α,15α-双羟DHEA P450酶基因的克隆及分析. (授权号, ZL201310570323.X ) [6] 一种利用亚麻刺盘孢高效合成15α-羟基-黄体酮的方法. (授权号: ZL201410683239.3) [7] 一种利用赤霉菌合成11α,15α-diOH-沃氏氧化物的方法,(授权号:ZL201610017919.0) [8] 一种利用赤霉菌CA3-1合成11α,15α-diOH-坎利酮的方法,(授权号:ZL201610017983.9) [9] 一株高产多糖的菌株及利用该菌株发酵生产多糖的方法. 授权号, ZL201210054829.0. 【主要科研奖励】 [1] 高等学校科学研究技术发明二等奖,2018, 排名2. [2] 全国商业联合会科技进步奖一等奖,2018,排名1 |