科学研究情况简介
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【近年来获得的学术科研成果】
已获批国际专利2项，中国专利1项，正在申请国际专利1项，已公开中国专利20余项。
【主要授权和公开专利】
1、Enzymatic synthesis of sulfated polysaccharides，国际发明专利，WO2006124801
2、Purified and isolated heparin sulfate 3-O-sulfotransferase isoform 5 nucleic acids and therapeutic and screening methods using SAME isoform 5 nucleic acids and therapeutic and screening methods using SAME，国际发明专利，WO2004005475。
3、一种制备肝素修饰的金纳米粒子的方法，发明专利号，ZL200910024843.4
【近年来主要研究课题】
1.新型生物医用材料的研制, 新世纪优秀人才支持计划。
2.酶法选择性改造肝素结构制备肝素衍生物及其抗凝血活性研究，教育部博士点基金。
3.表面原位合成类肝素以提高生物材料血液相溶性研究，国家自然科学基金。
4.蛋白质吸附行为与血液相容行生物高分子材料的制备，973前期专项子课题。
5.新型抗凝血硫酸乙酰肝素的生物合成及生物活性，教育部回国留学人员启动基金。
【近年来主要论文代表作】
1. C. Deng, H. Fu, L. Teng, Z. Hu, X. Xu, J. Chen*, T. Ren, Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata, International Journal of Biological Macromolecules, 2013, 61, 453-458.
2. J. Chen, J. Chen, J. Song, Collagen-PVP hybrid based anti-inflammatory hydrogel for wound repairing,Journal of Controlled Release, 2013, 172: e125.
3. C. Deng, Z. Hu, H. Fu, M. Hu, X. Xu, J. Chen*, Chemical analysis and antioxidant activity in vitro of a β-D-glucan isolated from Dictyophora indusiata,International Journal of Biological Macromolecules, 2012, 51(1-2), 70-75.
4. Y. Liu, L. Liu, J. Chen, J. Li, G. Du, J. Chen, Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5,Bioprocess and Biosystems Engineering, 2012, 35(7), 1209-1218.
5. G. Lu, L. Kai, P. Zhao, Z. Xu, C. Deng, H. Zheng, J. Huang, J. Chen*, A novel in situ-formed hydrogel wound dressing by the photocross-linking of a chitosan derivative,Wound repair and regeneration, 2010, 18(1), 70-79.
6. X. Zhang, J. Huang, P. Chang, J. Li, Y. Chen, D. Wang, J. Yu, J. Chen, Structure and properties of polysaccharide nanocrystal-doped supramolecular hydrogels based on Cyclodextrin inclusion,Polymer, 2010, 51(19), 4398-4407.
7. P. Xu, G. Guo, J. Huang, S. Zhou, D. Wang, J. Yu, J. Chen, Alginate-based ferrofluid and magnetic microsphere thereof,International Journal of Biological Macromolecules, 2010, 47(5), 654-660.
8. J. Wang, X. Xu, H. Zheng, J. Li, C. Deng, Z. Xu, J. Chen*, Structural Characterization, Chain Conformation, and Morphology of aβ-(1→3)-D-Glucan Isolated from the Fruiting Body of Dictyophora indusiata,Journal of Agricultural and Food Chemistry, 2009, 57(13), 5918-5924.
9. K. Ling, F. Zheng, J. Li, R. Tang, J. Huang, Y. Xu, H. Zheng, J.Chen*, Effects of substitution degree of photoreactive group on the properties on the UV-fabricated chitosan scaffold,Journal of Biomedical Material Research (A), 2008, 87(1), 52-61.
10. J. Chen, J. Courtney, J. Liu, Using an enzymatic combinatorial approach to identify anticoagulant heparan structures,Chemistry and Biology, 2007, 14, 986-993.
11. J. Chen, F.Y. Avci, E.M. Munoz et. al., Enzymatic redesigning of biologically active heparan sulfate, Journal of Biological Chemistry, 2005, 280, 42817-25.
12. G. Xia, J. Chen, V. Tiwari, W. Ju et. al., Heparan sulfate 3-O-sulfotransferase isoform 5 generates both an antithrombin-III binding site and an entry receptor for herpes simplex virus, type 1,Journal of Biological Chemistry,2002, 277, 37912-37919.
13. L.M McDowell，B.A. Frazier，D.R. Studelska，K. Giljum，J. Chen et. al., Inhibition or activation of apert syndrome FGFR2(S252W) signaling by specific glycosaminoglycans, Journal of Biological Chemistry, 2006, 281, 6924-6930.
14. A. Neigishi, J. Chen, D.M. MacCarty, R.J. Samulski et. al., Analysis of the interaction between adeno-associated virus and heparan sulfate using atomic force microscopy, Glycobiology, 2004, 14, 969-977.
15. J. Chen, M.B.
Duncan
, K. carrick, R.M. Pope et. al., Biosynthesis of 3-O-sulfotransferase heparan sulfate: unique substrate specificity of 3-O-sulfotransferase isoform 5, Glycobiology,2003, 13, 785-794.
16. J. Chen and J. Liu, Characterization of the structure of antithrombin-binding heparan sulfate generated by heparan sulfate 3-O-sulfotransferase 5, Biochim Biophys Acta, 2005, 1725, 190-200.
17. M.B. Duncan, J Chen, J.P. Krise, J. Liu, The biosynthesis of anticoagulant heparan sulfate by the heparan sulfate 3-O-sulfotransferase isoform 5, Biochim Biophys Acta, 2004, 1671, 34-43.
18. V. Tiwari, C. Clement, M.B. Duncan, J. Chen, et. al., A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1,Journal of GeneralVirology, 2004, 85, 805-809.
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