Projects [1] Analysis on the biosynthetic pathway and regulation mechanism of the polysaccharide BMPS produced by Bacillus mucilaginosus. Natural science foundation of China (No: 31300026), 2014.01-2016.12 [2] Research on the metabolic pathway and regulation of microbial expolysaccharide BMPS. Jiangsu Provincial Natural Science Foundation (No: BK2012117), 2012.07-2015.06 [3] Study on the efficient biotransformation mechanism and regulation of steroidal drugs intermediates 3β,7α,15α-trihydroxy-5-androstene-17-one. Fundamental Research Funds for the Central Universities (No: JUSRP111A50), 2013.01-2015.12 [4] Bioconversion technology of steroid compounds. National High-Tech R&D Program (No.2011AA02A211-05), 2011.09-2016.08 Recent Publications [1] Genome shuffling of Colletotrichum lini for improving 3β,7α,15α-trihydroxy-5-androsten-17-one production from dehydroepiandrosterone. Journal of Industrial Microbiology and Biotechnology, 2017, 1-11. [2] Purification and characterization of a glycosidase with hydrolyzing multi-3-O-glycosides of spirostanol saponin activity from Gibberella intermedia. Journal of Molecular Catalysis B: Enzymatic, 2016, 128: 46-51. [3] Enhanced 3β,7α,15α-trihydroxy-5-androsten-17-one production from dehydroepiandrosterone by Colletotrichum lini ST-1 resting cells with Tween-80. Applied Biochemistry and Biotechnology, 2016, 178:91-100. [4] Production of 7α,15α-diOH-DHEA from dehydroepiandrosterone by Colletotrichum lini ST-1 through integrating glucose-feeding with multi-step substrate addition strategy. Bioprocess and Biosystems Engineering, 2016, 39:1-8. [5] Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration. Green Chemistry, 2016, 18,1774-1784. [6] Improvement of NADPH-dependent P450-mediated biotransformation of 7α,15α-diOH-DHEA from DHEA by a dual cosubstrate-coupled system. Steroids, 2015,101: 15-20. [7] The efficient production of 3β,7α,15α-trihydroxy-5-androsten-17-one from dehydroepiandrosterone by Gibberella intermedia. Appl Biochem Biotechnol, 2014, 174:2960-2971. [8] Efficient hydroxylation of functionalized steroids by Colletotrichum lini ST-1. Journal of Molecular Catalysis B: Enzymatic, 2015, 120: 111-118. [9] Enhanced biotransformation of dehydroepiandrosterone to 3β,7α,15α‑trihydroxy‑5‑androsten‑17‑one with Gibberella intermedia CA3-1 by natural oils addition. J Ind Microbiol Biotechnol, 2014, 41:1497-1504. [10] Enhancement of steroid hydroxylation yield from dehydroepiandrosterone by cyclodextrin complexation technique. Steroids, 2014, 84:70-77. [11] Improvement of welan gum production and redistribution of metabolic flux under pH control process in Alcaligenes sp. CGMCC2428. Biotechnology and Bioprocess Engineering, 2013,18: 399-405. [12] Improvement of the steroid dihydroxylation efficiency from dehydroepiandrosterone using a substrate pre-induction biotransformation process. Biotechnology and Bioprocess Engineering, 2013,18: 486-490. [13] Optimization of exopolysaccharide welan gum production by Alcaligenes sp. CGMCC2428 with Tween-40 using response surface methodology. Carbohydrate Polymers, 2012, 87(2):1363-1368. [14] Effect of dissolved oxygen and shear stress on the synthesis and molecular weight of welan gum produced by Alcaligenes sp. CGMCC2428. Process Biochemistry. 2011, 46(5): 1172-1178. [15] Biosynthetic pathway of sugar nucleotides essential for welan gum production in Alcaligenes sp. CGMCC2428. Applied Microbiology and Biotechnology. 2010, 86(1): 295-303. Patents [1] A strain of high efficient conversion of plant saponins to diosgenin and its applications, ZL201210167132.4 [2] A strain of high efficient conversion of dehydroepiandrosterone and its applications, ZL201110438752.2 [3] A method for hydroxylation of dehydroepiandrosterone using Colletotrichum lini, ZL2012104167406.X [4] A cleaner production process for diosgenin in Dioscorea zingiberensis C. H. Wright based on the enzymatic extraction and microwave acidolysis, ZL201110285328.9 [5] A strain of high efficient conversion of Dioscorea zingiberensis C. H. Wright and its application, ZL201310040600.6 [6] A method for high efficient synthesis of 3β,7α-dihydroxy-5-androsten-17-one using Colletotrichum lini, ZL201310570312.1 [7] A P450 enzyme gene cloning for dihydroxylation of DHEA and analysis, ZL201310570323.X |
