科学研究情况简介 |
【近年来获得的学术科研成果】 主要研究方向为：功能性食品添加剂(低聚木糖、甘露糖、苯乳酸、阿魏酸等)及手性药物或精细化工中间体的生物合成及工艺开发；新型酶资源的挖掘、理性改造及应用研究；手性药物或精细化工中间体(β-卤代醇、环氧化物、邻二醇等)的酶法制备。 完成国家、省、校级和企业委托科研项目30余项；指导国家大学生创新性实验计划项目3项、江苏省研究生创新计划项目10余项；江南大学博士研究生科学研究基金项目4项。获全国、协会、市级科技和教学成果奖10余项；获授权或申请技术发明专利30余项；主编和参编论著4部，发表学术论文100余篇，其中Biotechnol Biofuels、Biotechnol Bioeng、J Agric Food Chem、Appl Microbiol Biotechnol、Int J Biol Macromol及J Biotechnol等SCI期刊收录论文50余篇。获得“中国石油与化学工业协会科技进步奖二等奖”、“无锡市具有突出贡献的中青年专家”、“无锡市科学技术奖二等奖”、“江南大学青蓝工程优秀青年骨干教师”和“江南大学2003-2005年度优秀教育工作者”等称号。 【近年来主要研究课题】 1.国家自然科学基金面上项目 (21676117)，基于理性设计的新型菜豆环氧化物水解酶定向改造，2017.01-2020.12，在研，主持； 2.国家自然科学基金面上项目 (20776061)，碱性脂肪酶热稳定性的生物信息学分析及定点突变，2008.01-2010.12，已结题，主持； 3.国家自然科学基金面上项目 (31271811)，基于理性化设计的β-甘露聚糖酶分子定向改造，2013.01-2016.12，已结题，参加； 4.国家自然科学青年基金项目 (31101229)，宇佐美曲霉木聚糖酶耐热/耐碱性的分子改造，2012.01-2014.12，已结题，参加。 【近年来主要论文代表作】 1. C Li, BC Hu, D Hu, XF Xu, XC Zong, JP Li, MC Wu*. Stereoselective ring-opening of styrene oxide at elevated concentration byPhaseolus vulgarisepoxide hydrolase,PvEH2, in the organic/aqueous biphasic system.Catalysis communications, 2019, 123: 1–5. 2. C Li, J Zhao, D Hu, BC Hu, R Wang,MC Wu*, J Zang. Multiple site-directed mutagenesis of aPhaseolus vulgaris epoxide hydrolase to improve its catalytic performance towardsp-chlorostyrene oxide based on the computer-aided re-design.International Journal of Biological Macromolecules, 2019, 121: 326–332. 3. JF Li, XQ Li, Y Liu, FJ Yuan, T Zhang,MC Wu*, JR Zhang. Directed modification of l-LcLDH1, an l-lactate dehydrogenase fromLactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid.Journal of Biotechnology, 2018, 281: 193–198. 4. BC Hu, C Li, R Wang, XC Zong, JP Li, JF Li,MC Wu*, Improvement in the activity and enantioconvergency ofPvEH3, an epoxide hydrolase fromPhaseolus vulgaris, forp-chlorostyrene oxide by sitesaturation mutagenesis,Catalysis communications, 2018, 117: 9–13. 5. D Hu, CD Tang, C Li, TT Kan, XL Shi, L Feng,MC Wu*. Stereoselective hydrolysis of epoxides by reVrEH3, a novelvigna radiataepoxide hydrolase with high enantioselectivity or high and complementary regioselectivity.Journal of Agricultural and Food Chemistry, 2017, 65(45): 9861–9870. 6. D Hu, R Wang, XL Shi, HH Ye, Q Wu,MC Wu*, JJ Chu. Kinetic resolution of racemic styrene oxide at a high concentration by recombinantAspergillus usamiiepoxide hydrolase in an n-hexanol/buffer biphasic system.Journal of Biotechnology, 2016, 236: 152–158. 7. YH Dong, JF Li, D Hu, X Yin, CJ Wang, SH Tang, MC Wu*. Replacing a piece of loop-structure in the substrate-binding groove ofAspergillus usamiiβ-mannanase, AuMan5A, to improve its enzymatic properties by rational design.Applied Microbiology and Biotechnology, 2016, 100(9): 3989–3998. 8. D Hu, CD Tang, B Yang, JC Liu, T Yu, C Deng,MC Wu*. Expression of a novel epoxide hydrolase ofAspergillus usamiiE001 inEscherichia coli and its performance in resolution of racemic styrene oxide.Journal of Industrial Microbiology & Biotechnology, 2015, 42(5): 671–680. 9. X Yin, JF Li JF, CJ Wang, D Hu, Q Wu, Y Gu,MC Wu*. Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, fromAspergillus usamiiby iterative saturation mutagenesis.Applied Microbiology and Biotechnology, 2015, 99(23): 10047–10056. 10. HM Zhang, JF Li, JQ Wang, YJ Yang,MC Wu*. Determinants for the improved thermostability of a mesophilic family 11 xylanase predicted by computational methods.Biotechnology for Biofuels, 2014, 7(1):3. 【主要授权国家发明专利】 1.一种双酶催化外消旋环氧苯乙烷制备(R)-苯乙二醇的方法.授权号: ZL201610926649.5 2.一种双酶法不对称制备(R)-2-氯-1-(3-氯苯基)乙醇的应用.授权号: ZL201410759167.6 3.一种添加二硫键以提高阿魏酸酯酶A热稳定性的方法.授权号: ZL201410758084.5 4.一种环氧化物水解酶突变体的设计及制备方法.授权号: ZL201410752769.9 5.一种N端替换提高米曲霉木聚糖酶(AoXyn11A)热稳定性的方法.授权号: ZL201410757519.4 |
